Analysis of cases of brucellosis in humans and molecular-biological characteristics of <i>Brucella melitensis</i> strains in regions of South European Russia with a high brucellosis incidence

Introduction. To improve the epidemiological surveillance of brucellosis in Russia, a detailed analysis incidence and characteristics Brucella melitensis strains circulating territories country where there is persistent epizootic problem with required.Purpose study. study current trends epidemic process perform molecular isolates from Southern North Caucasian Federal Districts long lasting unfavorable situation — Republics Dagestan (RD), Kalmykia (RK) Stavropol Krai (SK).Materials methods. The Caucasus District was carried out on basis data Rospotrebnadzor Departments for period 2011–2020. Total 56 cultures melitensis, isolated 1999–2019 patients that permanently resided RD, RK SK were studied.Results. key factor contributing to manifestations south European part Russia persistence foci cattle small ruminants. leading concomitant factors are as follows: presence region "hidden" foci, uncontrolled production food products animal husbandry its illegal sale customers, unauthorized movement animals livestock products, untimely delivery (concealment) sick stamping out. Results B. indicate circulation mixed population brucella, which generally characteristic entire region, without pronounced confinement separate administrative territories. At same time, studied sample can be divided into two groups (72% 28% strains) based MLVA-16 genotypes by variability Bruce 19 locus, makes it possible associate territory their isolation.Discussion. obtained make scientifically substantiate possibility considering affected single common active anthropurgic focus mixed, but typical strains.